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algorithms image lab 3 0 build 11 bio rad laboratories n a imagej 1 53e nih  (Bio-Rad)


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    Bio-Rad algorithms image lab 3 0 build 11 bio rad laboratories n a imagej 1 53e nih
    Algorithms Image Lab 3 0 Build 11 Bio Rad Laboratories N A Imagej 1 53e Nih, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 36344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/algorithms+image+lab+3+0+build+11+bio+rad+laboratories+n+a+imagej+1+53e+nih/Image+Lab+Software/pm35427476-199-76-82
    Average 99 stars, based on 36344 article reviews
    algorithms image lab 3 0 build 11 bio rad laboratories n a imagej 1 53e nih - by Bioz Stars, 2026-09
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    AST Assay:

    Article Title: Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GAPDH Abcam Cat# Ab181602; RRID: AB_2630358 Rabbit anti-HNF1 alpha Abcam Cat# Ab272693 Rabbit anti-ANGPTL3 Abcam Cat# Ab126718; RRID: AB_11128146 Mouse anti-b-actin Sigma Aldrich Cat# A5441-100ul; RRID: AB_476744 Rabbit anti-PCSK9 Abcam Cat# ab181142 Rabbit anti-LDLR Proteintech Cat# 10785-1-LP; RRID: AB_2281164 Histone H3 Sigma Aldrich Cat# H9289; RRID: AB_1079063 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat# ab97051; RRID: AB_10679369 Rabbit anti-HNF-1a Santa Cruz Cat# sc-393925 X Biological samples Hamster Plasma Shanghai Institute of Materia Medica N/A Rat Plasma Shanghai Institute of Materia Medica N/A Monkey Plasma Sichuan Primed Shines Biotech Co., Ltd. N/A Human Plasma The First Hospital of Jilin University ClinicalTrials.gov, NCT04927221 Chemicals, peptides, and recombinant proteins DiI-Low Density Lipoprotein YEASEN Cat# 20614ES PageRuler Prestained Protein Ladder Invitrogen Cat# 26616 Protease Inhibitor Cocktail Set III Calbiochem Cat# 539134-1ML SYBR PCR Master mix Bio-Rad Cat# 1725124 dNTP Mixture Takara Cat# 4030 TRIzol Reagent Thermo Fisher Cat# 15596018 M-MLV Reverse Transcriptase Promega Cat# M1705 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K Lipofectamine 3000 Invitrogen Cat# L3000-015 Protease inhibitor cocktail Bimake Cat# B14011 Anti-Flag M2 beads Sigma-Aldrich Cat# F2426 NP40 BBI Life Sciences Cooperation Cat# 9016–45-9 Octet Red96 instrument Pall ForteBio N/A GST beads YESEN Cat# 20507ES50 RNA oligo GenePharma HNF1A-homo-979 Zwittergent Sigma-Aldrich Cat# 693015-5GM EnzChek Lipase Substrate, green fluorescent Invitrogen Cat# E33955 Bovine Serum Albumin, BSA, Fat Acid Free Equitech-Bio Cat# BAH66-0050 Atorvastatin Calcium ZaiQi Bio-Tech Cat# 134523-03-8 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K RIPA Lysis Buffer Beyotime Cat# P0013B Rat tail tendon collagen type I shengyou Cat# 200110-10 Collagenase IV YEASEN Cat# 40510ES60 Critical commercial assays Low Density Lipoprotein (LDL) Cholesterol Assay Kit Wako A.20.991-39891; A.20.991-39991 High Density Lipoprotein (HDL) Cholesterol Assay Kit Wako A.20.991-09001; A.20.997-09101 Total Cholesterol Assay Kit Shino A.19.A808310; A.19.A808320 Triglyceride Assay Kit Maccura A.29.CH0101151 ALT Assay Kit Shino A.19.A800680; A.19.A800690 (Continued on next page) Cell Metabolism 34, 667–680.e1–e6, May 3, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER AST Assay Kit Shino A.19.A800660; A.19.A800670 ChIP kit CST Cat# 9003 Enhanced BCA Protein Assay Kit Beyotime Cat# P0010S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat# P0027 ECL kit Bio-Rad Cat# 170-5061 Deposited data Raw RNA–seq data This paper GEO: GSE196191 Experimental models: Cell lines HepG2 National Collection of Authenticated Cell Cultures TCHu 72 HEK293 National Collection of Authenticated Cell Cultures GNHu 43 LO2 China Pharmaceutical University N/A Software and algorithms Image Lab 3.0 build 11 Bio-Rad Laboratories N/A ImageJ 1.53e NIH http://imagej.nih.gov/ij Analyst 1.6.3 software SCIEX N/A R (v4.1.1) CRAN https://www.r-project.org/ Samtools v1.10 Li et al., 2009a, 2009b https://www.htslib.org/ HISAT2 v2.2.1 Kim et al., 2019 http://daehwankimlab.github.io/ hisat2/ Feature Counts v2.0.0 Liao et al., 2014 http://subread.sourceforge.net/ Adobe Illustrator CS5 Adobe https://www.adobe.com/cn/ products/illustrator.html Other Fetal bovine serum GIBCO Cat# 10270106 Trypsin GIBCO Cat# 25200056 DMEM Hyclone Cat# SH30022.01B RPMI 1640 GIBCO Cat# 11875-093 Opti-MEM GIBCO Cat# 31985062 Trypsin-EDTA (0.25%), phenol red Invitrogen Cat# 25200056 Normal chow (NC) diet Shanghai shilin Bio. ..

    Chromatin Immunoprecipitation:

    Article Title: Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GAPDH Abcam Cat# Ab181602; RRID: AB_2630358 Rabbit anti-HNF1 alpha Abcam Cat# Ab272693 Rabbit anti-ANGPTL3 Abcam Cat# Ab126718; RRID: AB_11128146 Mouse anti-b-actin Sigma Aldrich Cat# A5441-100ul; RRID: AB_476744 Rabbit anti-PCSK9 Abcam Cat# ab181142 Rabbit anti-LDLR Proteintech Cat# 10785-1-LP; RRID: AB_2281164 Histone H3 Sigma Aldrich Cat# H9289; RRID: AB_1079063 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat# ab97051; RRID: AB_10679369 Rabbit anti-HNF-1a Santa Cruz Cat# sc-393925 X Biological samples Hamster Plasma Shanghai Institute of Materia Medica N/A Rat Plasma Shanghai Institute of Materia Medica N/A Monkey Plasma Sichuan Primed Shines Biotech Co., Ltd. N/A Human Plasma The First Hospital of Jilin University ClinicalTrials.gov, NCT04927221 Chemicals, peptides, and recombinant proteins DiI-Low Density Lipoprotein YEASEN Cat# 20614ES PageRuler Prestained Protein Ladder Invitrogen Cat# 26616 Protease Inhibitor Cocktail Set III Calbiochem Cat# 539134-1ML SYBR PCR Master mix Bio-Rad Cat# 1725124 dNTP Mixture Takara Cat# 4030 TRIzol Reagent Thermo Fisher Cat# 15596018 M-MLV Reverse Transcriptase Promega Cat# M1705 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K Lipofectamine 3000 Invitrogen Cat# L3000-015 Protease inhibitor cocktail Bimake Cat# B14011 Anti-Flag M2 beads Sigma-Aldrich Cat# F2426 NP40 BBI Life Sciences Cooperation Cat# 9016–45-9 Octet Red96 instrument Pall ForteBio N/A GST beads YESEN Cat# 20507ES50 RNA oligo GenePharma HNF1A-homo-979 Zwittergent Sigma-Aldrich Cat# 693015-5GM EnzChek Lipase Substrate, green fluorescent Invitrogen Cat# E33955 Bovine Serum Albumin, BSA, Fat Acid Free Equitech-Bio Cat# BAH66-0050 Atorvastatin Calcium ZaiQi Bio-Tech Cat# 134523-03-8 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K RIPA Lysis Buffer Beyotime Cat# P0013B Rat tail tendon collagen type I shengyou Cat# 200110-10 Collagenase IV YEASEN Cat# 40510ES60 Critical commercial assays Low Density Lipoprotein (LDL) Cholesterol Assay Kit Wako A.20.991-39891; A.20.991-39991 High Density Lipoprotein (HDL) Cholesterol Assay Kit Wako A.20.991-09001; A.20.997-09101 Total Cholesterol Assay Kit Shino A.19.A808310; A.19.A808320 Triglyceride Assay Kit Maccura A.29.CH0101151 ALT Assay Kit Shino A.19.A800680; A.19.A800690 (Continued on next page) Cell Metabolism 34, 667–680.e1–e6, May 3, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER AST Assay Kit Shino A.19.A800660; A.19.A800670 ChIP kit CST Cat# 9003 Enhanced BCA Protein Assay Kit Beyotime Cat# P0010S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat# P0027 ECL kit Bio-Rad Cat# 170-5061 Deposited data Raw RNA–seq data This paper GEO: GSE196191 Experimental models: Cell lines HepG2 National Collection of Authenticated Cell Cultures TCHu 72 HEK293 National Collection of Authenticated Cell Cultures GNHu 43 LO2 China Pharmaceutical University N/A Software and algorithms Image Lab 3.0 build 11 Bio-Rad Laboratories N/A ImageJ 1.53e NIH http://imagej.nih.gov/ij Analyst 1.6.3 software SCIEX N/A R (v4.1.1) CRAN https://www.r-project.org/ Samtools v1.10 Li et al., 2009a, 2009b https://www.htslib.org/ HISAT2 v2.2.1 Kim et al., 2019 http://daehwankimlab.github.io/ hisat2/ Feature Counts v2.0.0 Liao et al., 2014 http://subread.sourceforge.net/ Adobe Illustrator CS5 Adobe https://www.adobe.com/cn/ products/illustrator.html Other Fetal bovine serum GIBCO Cat# 10270106 Trypsin GIBCO Cat# 25200056 DMEM Hyclone Cat# SH30022.01B RPMI 1640 GIBCO Cat# 11875-093 Opti-MEM GIBCO Cat# 31985062 Trypsin-EDTA (0.25%), phenol red Invitrogen Cat# 25200056 Normal chow (NC) diet Shanghai shilin Bio. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GAPDH Abcam Cat# Ab181602; RRID: AB_2630358 Rabbit anti-HNF1 alpha Abcam Cat# Ab272693 Rabbit anti-ANGPTL3 Abcam Cat# Ab126718; RRID: AB_11128146 Mouse anti-b-actin Sigma Aldrich Cat# A5441-100ul; RRID: AB_476744 Rabbit anti-PCSK9 Abcam Cat# ab181142 Rabbit anti-LDLR Proteintech Cat# 10785-1-LP; RRID: AB_2281164 Histone H3 Sigma Aldrich Cat# H9289; RRID: AB_1079063 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat# ab97051; RRID: AB_10679369 Rabbit anti-HNF-1a Santa Cruz Cat# sc-393925 X Biological samples Hamster Plasma Shanghai Institute of Materia Medica N/A Rat Plasma Shanghai Institute of Materia Medica N/A Monkey Plasma Sichuan Primed Shines Biotech Co., Ltd. N/A Human Plasma The First Hospital of Jilin University ClinicalTrials.gov, NCT04927221 Chemicals, peptides, and recombinant proteins DiI-Low Density Lipoprotein YEASEN Cat# 20614ES PageRuler Prestained Protein Ladder Invitrogen Cat# 26616 Protease Inhibitor Cocktail Set III Calbiochem Cat# 539134-1ML SYBR PCR Master mix Bio-Rad Cat# 1725124 dNTP Mixture Takara Cat# 4030 TRIzol Reagent Thermo Fisher Cat# 15596018 M-MLV Reverse Transcriptase Promega Cat# M1705 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K Lipofectamine 3000 Invitrogen Cat# L3000-015 Protease inhibitor cocktail Bimake Cat# B14011 Anti-Flag M2 beads Sigma-Aldrich Cat# F2426 NP40 BBI Life Sciences Cooperation Cat# 9016–45-9 Octet Red96 instrument Pall ForteBio N/A GST beads YESEN Cat# 20507ES50 RNA oligo GenePharma HNF1A-homo-979 Zwittergent Sigma-Aldrich Cat# 693015-5GM EnzChek Lipase Substrate, green fluorescent Invitrogen Cat# E33955 Bovine Serum Albumin, BSA, Fat Acid Free Equitech-Bio Cat# BAH66-0050 Atorvastatin Calcium ZaiQi Bio-Tech Cat# 134523-03-8 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K RIPA Lysis Buffer Beyotime Cat# P0013B Rat tail tendon collagen type I shengyou Cat# 200110-10 Collagenase IV YEASEN Cat# 40510ES60 Critical commercial assays Low Density Lipoprotein (LDL) Cholesterol Assay Kit Wako A.20.991-39891; A.20.991-39991 High Density Lipoprotein (HDL) Cholesterol Assay Kit Wako A.20.991-09001; A.20.997-09101 Total Cholesterol Assay Kit Shino A.19.A808310; A.19.A808320 Triglyceride Assay Kit Maccura A.29.CH0101151 ALT Assay Kit Shino A.19.A800680; A.19.A800690 (Continued on next page) Cell Metabolism 34, 667–680.e1–e6, May 3, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER AST Assay Kit Shino A.19.A800660; A.19.A800670 ChIP kit CST Cat# 9003 Enhanced BCA Protein Assay Kit Beyotime Cat# P0010S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat# P0027 ECL kit Bio-Rad Cat# 170-5061 Deposited data Raw RNA–seq data This paper GEO: GSE196191 Experimental models: Cell lines HepG2 National Collection of Authenticated Cell Cultures TCHu 72 HEK293 National Collection of Authenticated Cell Cultures GNHu 43 LO2 China Pharmaceutical University N/A Software and algorithms Image Lab 3.0 build 11 Bio-Rad Laboratories N/A ImageJ 1.53e NIH http://imagej.nih.gov/ij Analyst 1.6.3 software SCIEX N/A R (v4.1.1) CRAN https://www.r-project.org/ Samtools v1.10 Li et al., 2009a, 2009b https://www.htslib.org/ HISAT2 v2.2.1 Kim et al., 2019 http://daehwankimlab.github.io/ hisat2/ Feature Counts v2.0.0 Liao et al., 2014 http://subread.sourceforge.net/ Adobe Illustrator CS5 Adobe https://www.adobe.com/cn/ products/illustrator.html Other Fetal bovine serum GIBCO Cat# 10270106 Trypsin GIBCO Cat# 25200056 DMEM Hyclone Cat# SH30022.01B RPMI 1640 GIBCO Cat# 11875-093 Opti-MEM GIBCO Cat# 31985062 Trypsin-EDTA (0.25%), phenol red Invitrogen Cat# 25200056 Normal chow (NC) diet Shanghai shilin Bio. ..

    Protein Extraction:

    Article Title: Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GAPDH Abcam Cat# Ab181602; RRID: AB_2630358 Rabbit anti-HNF1 alpha Abcam Cat# Ab272693 Rabbit anti-ANGPTL3 Abcam Cat# Ab126718; RRID: AB_11128146 Mouse anti-b-actin Sigma Aldrich Cat# A5441-100ul; RRID: AB_476744 Rabbit anti-PCSK9 Abcam Cat# ab181142 Rabbit anti-LDLR Proteintech Cat# 10785-1-LP; RRID: AB_2281164 Histone H3 Sigma Aldrich Cat# H9289; RRID: AB_1079063 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat# ab97051; RRID: AB_10679369 Rabbit anti-HNF-1a Santa Cruz Cat# sc-393925 X Biological samples Hamster Plasma Shanghai Institute of Materia Medica N/A Rat Plasma Shanghai Institute of Materia Medica N/A Monkey Plasma Sichuan Primed Shines Biotech Co., Ltd. N/A Human Plasma The First Hospital of Jilin University ClinicalTrials.gov, NCT04927221 Chemicals, peptides, and recombinant proteins DiI-Low Density Lipoprotein YEASEN Cat# 20614ES PageRuler Prestained Protein Ladder Invitrogen Cat# 26616 Protease Inhibitor Cocktail Set III Calbiochem Cat# 539134-1ML SYBR PCR Master mix Bio-Rad Cat# 1725124 dNTP Mixture Takara Cat# 4030 TRIzol Reagent Thermo Fisher Cat# 15596018 M-MLV Reverse Transcriptase Promega Cat# M1705 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K Lipofectamine 3000 Invitrogen Cat# L3000-015 Protease inhibitor cocktail Bimake Cat# B14011 Anti-Flag M2 beads Sigma-Aldrich Cat# F2426 NP40 BBI Life Sciences Cooperation Cat# 9016–45-9 Octet Red96 instrument Pall ForteBio N/A GST beads YESEN Cat# 20507ES50 RNA oligo GenePharma HNF1A-homo-979 Zwittergent Sigma-Aldrich Cat# 693015-5GM EnzChek Lipase Substrate, green fluorescent Invitrogen Cat# E33955 Bovine Serum Albumin, BSA, Fat Acid Free Equitech-Bio Cat# BAH66-0050 Atorvastatin Calcium ZaiQi Bio-Tech Cat# 134523-03-8 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K RIPA Lysis Buffer Beyotime Cat# P0013B Rat tail tendon collagen type I shengyou Cat# 200110-10 Collagenase IV YEASEN Cat# 40510ES60 Critical commercial assays Low Density Lipoprotein (LDL) Cholesterol Assay Kit Wako A.20.991-39891; A.20.991-39991 High Density Lipoprotein (HDL) Cholesterol Assay Kit Wako A.20.991-09001; A.20.997-09101 Total Cholesterol Assay Kit Shino A.19.A808310; A.19.A808320 Triglyceride Assay Kit Maccura A.29.CH0101151 ALT Assay Kit Shino A.19.A800680; A.19.A800690 (Continued on next page) Cell Metabolism 34, 667–680.e1–e6, May 3, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER AST Assay Kit Shino A.19.A800660; A.19.A800670 ChIP kit CST Cat# 9003 Enhanced BCA Protein Assay Kit Beyotime Cat# P0010S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat# P0027 ECL kit Bio-Rad Cat# 170-5061 Deposited data Raw RNA–seq data This paper GEO: GSE196191 Experimental models: Cell lines HepG2 National Collection of Authenticated Cell Cultures TCHu 72 HEK293 National Collection of Authenticated Cell Cultures GNHu 43 LO2 China Pharmaceutical University N/A Software and algorithms Image Lab 3.0 build 11 Bio-Rad Laboratories N/A ImageJ 1.53e NIH http://imagej.nih.gov/ij Analyst 1.6.3 software SCIEX N/A R (v4.1.1) CRAN https://www.r-project.org/ Samtools v1.10 Li et al., 2009a, 2009b https://www.htslib.org/ HISAT2 v2.2.1 Kim et al., 2019 http://daehwankimlab.github.io/ hisat2/ Feature Counts v2.0.0 Liao et al., 2014 http://subread.sourceforge.net/ Adobe Illustrator CS5 Adobe https://www.adobe.com/cn/ products/illustrator.html Other Fetal bovine serum GIBCO Cat# 10270106 Trypsin GIBCO Cat# 25200056 DMEM Hyclone Cat# SH30022.01B RPMI 1640 GIBCO Cat# 11875-093 Opti-MEM GIBCO Cat# 31985062 Trypsin-EDTA (0.25%), phenol red Invitrogen Cat# 25200056 Normal chow (NC) diet Shanghai shilin Bio. ..

    RNA Sequencing:

    Article Title: Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GAPDH Abcam Cat# Ab181602; RRID: AB_2630358 Rabbit anti-HNF1 alpha Abcam Cat# Ab272693 Rabbit anti-ANGPTL3 Abcam Cat# Ab126718; RRID: AB_11128146 Mouse anti-b-actin Sigma Aldrich Cat# A5441-100ul; RRID: AB_476744 Rabbit anti-PCSK9 Abcam Cat# ab181142 Rabbit anti-LDLR Proteintech Cat# 10785-1-LP; RRID: AB_2281164 Histone H3 Sigma Aldrich Cat# H9289; RRID: AB_1079063 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat# ab97051; RRID: AB_10679369 Rabbit anti-HNF-1a Santa Cruz Cat# sc-393925 X Biological samples Hamster Plasma Shanghai Institute of Materia Medica N/A Rat Plasma Shanghai Institute of Materia Medica N/A Monkey Plasma Sichuan Primed Shines Biotech Co., Ltd. N/A Human Plasma The First Hospital of Jilin University ClinicalTrials.gov, NCT04927221 Chemicals, peptides, and recombinant proteins DiI-Low Density Lipoprotein YEASEN Cat# 20614ES PageRuler Prestained Protein Ladder Invitrogen Cat# 26616 Protease Inhibitor Cocktail Set III Calbiochem Cat# 539134-1ML SYBR PCR Master mix Bio-Rad Cat# 1725124 dNTP Mixture Takara Cat# 4030 TRIzol Reagent Thermo Fisher Cat# 15596018 M-MLV Reverse Transcriptase Promega Cat# M1705 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K Lipofectamine 3000 Invitrogen Cat# L3000-015 Protease inhibitor cocktail Bimake Cat# B14011 Anti-Flag M2 beads Sigma-Aldrich Cat# F2426 NP40 BBI Life Sciences Cooperation Cat# 9016–45-9 Octet Red96 instrument Pall ForteBio N/A GST beads YESEN Cat# 20507ES50 RNA oligo GenePharma HNF1A-homo-979 Zwittergent Sigma-Aldrich Cat# 693015-5GM EnzChek Lipase Substrate, green fluorescent Invitrogen Cat# E33955 Bovine Serum Albumin, BSA, Fat Acid Free Equitech-Bio Cat# BAH66-0050 Atorvastatin Calcium ZaiQi Bio-Tech Cat# 134523-03-8 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K RIPA Lysis Buffer Beyotime Cat# P0013B Rat tail tendon collagen type I shengyou Cat# 200110-10 Collagenase IV YEASEN Cat# 40510ES60 Critical commercial assays Low Density Lipoprotein (LDL) Cholesterol Assay Kit Wako A.20.991-39891; A.20.991-39991 High Density Lipoprotein (HDL) Cholesterol Assay Kit Wako A.20.991-09001; A.20.997-09101 Total Cholesterol Assay Kit Shino A.19.A808310; A.19.A808320 Triglyceride Assay Kit Maccura A.29.CH0101151 ALT Assay Kit Shino A.19.A800680; A.19.A800690 (Continued on next page) Cell Metabolism 34, 667–680.e1–e6, May 3, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER AST Assay Kit Shino A.19.A800660; A.19.A800670 ChIP kit CST Cat# 9003 Enhanced BCA Protein Assay Kit Beyotime Cat# P0010S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat# P0027 ECL kit Bio-Rad Cat# 170-5061 Deposited data Raw RNA–seq data This paper GEO: GSE196191 Experimental models: Cell lines HepG2 National Collection of Authenticated Cell Cultures TCHu 72 HEK293 National Collection of Authenticated Cell Cultures GNHu 43 LO2 China Pharmaceutical University N/A Software and algorithms Image Lab 3.0 build 11 Bio-Rad Laboratories N/A ImageJ 1.53e NIH http://imagej.nih.gov/ij Analyst 1.6.3 software SCIEX N/A R (v4.1.1) CRAN https://www.r-project.org/ Samtools v1.10 Li et al., 2009a, 2009b https://www.htslib.org/ HISAT2 v2.2.1 Kim et al., 2019 http://daehwankimlab.github.io/ hisat2/ Feature Counts v2.0.0 Liao et al., 2014 http://subread.sourceforge.net/ Adobe Illustrator CS5 Adobe https://www.adobe.com/cn/ products/illustrator.html Other Fetal bovine serum GIBCO Cat# 10270106 Trypsin GIBCO Cat# 25200056 DMEM Hyclone Cat# SH30022.01B RPMI 1640 GIBCO Cat# 11875-093 Opti-MEM GIBCO Cat# 31985062 Trypsin-EDTA (0.25%), phenol red Invitrogen Cat# 25200056 Normal chow (NC) diet Shanghai shilin Bio. ..

    Software:

    Article Title: Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GAPDH Abcam Cat# Ab181602; RRID: AB_2630358 Rabbit anti-HNF1 alpha Abcam Cat# Ab272693 Rabbit anti-ANGPTL3 Abcam Cat# Ab126718; RRID: AB_11128146 Mouse anti-b-actin Sigma Aldrich Cat# A5441-100ul; RRID: AB_476744 Rabbit anti-PCSK9 Abcam Cat# ab181142 Rabbit anti-LDLR Proteintech Cat# 10785-1-LP; RRID: AB_2281164 Histone H3 Sigma Aldrich Cat# H9289; RRID: AB_1079063 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat# ab97051; RRID: AB_10679369 Rabbit anti-HNF-1a Santa Cruz Cat# sc-393925 X Biological samples Hamster Plasma Shanghai Institute of Materia Medica N/A Rat Plasma Shanghai Institute of Materia Medica N/A Monkey Plasma Sichuan Primed Shines Biotech Co., Ltd. N/A Human Plasma The First Hospital of Jilin University ClinicalTrials.gov, NCT04927221 Chemicals, peptides, and recombinant proteins DiI-Low Density Lipoprotein YEASEN Cat# 20614ES PageRuler Prestained Protein Ladder Invitrogen Cat# 26616 Protease Inhibitor Cocktail Set III Calbiochem Cat# 539134-1ML SYBR PCR Master mix Bio-Rad Cat# 1725124 dNTP Mixture Takara Cat# 4030 TRIzol Reagent Thermo Fisher Cat# 15596018 M-MLV Reverse Transcriptase Promega Cat# M1705 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K Lipofectamine 3000 Invitrogen Cat# L3000-015 Protease inhibitor cocktail Bimake Cat# B14011 Anti-Flag M2 beads Sigma-Aldrich Cat# F2426 NP40 BBI Life Sciences Cooperation Cat# 9016–45-9 Octet Red96 instrument Pall ForteBio N/A GST beads YESEN Cat# 20507ES50 RNA oligo GenePharma HNF1A-homo-979 Zwittergent Sigma-Aldrich Cat# 693015-5GM EnzChek Lipase Substrate, green fluorescent Invitrogen Cat# E33955 Bovine Serum Albumin, BSA, Fat Acid Free Equitech-Bio Cat# BAH66-0050 Atorvastatin Calcium ZaiQi Bio-Tech Cat# 134523-03-8 Lipoprotein lipase from bovine Sigma-Aldrich Cat# L2254-1K RIPA Lysis Buffer Beyotime Cat# P0013B Rat tail tendon collagen type I shengyou Cat# 200110-10 Collagenase IV YEASEN Cat# 40510ES60 Critical commercial assays Low Density Lipoprotein (LDL) Cholesterol Assay Kit Wako A.20.991-39891; A.20.991-39991 High Density Lipoprotein (HDL) Cholesterol Assay Kit Wako A.20.991-09001; A.20.997-09101 Total Cholesterol Assay Kit Shino A.19.A808310; A.19.A808320 Triglyceride Assay Kit Maccura A.29.CH0101151 ALT Assay Kit Shino A.19.A800680; A.19.A800690 (Continued on next page) Cell Metabolism 34, 667–680.e1–e6, May 3, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER AST Assay Kit Shino A.19.A800660; A.19.A800670 ChIP kit CST Cat# 9003 Enhanced BCA Protein Assay Kit Beyotime Cat# P0010S Nuclear and Cytoplasmic Protein Extraction Kit Beyotime Cat# P0027 ECL kit Bio-Rad Cat# 170-5061 Deposited data Raw RNA–seq data This paper GEO: GSE196191 Experimental models: Cell lines HepG2 National Collection of Authenticated Cell Cultures TCHu 72 HEK293 National Collection of Authenticated Cell Cultures GNHu 43 LO2 China Pharmaceutical University N/A Software and algorithms Image Lab 3.0 build 11 Bio-Rad Laboratories N/A ImageJ 1.53e NIH http://imagej.nih.gov/ij Analyst 1.6.3 software SCIEX N/A R (v4.1.1) CRAN https://www.r-project.org/ Samtools v1.10 Li et al., 2009a, 2009b https://www.htslib.org/ HISAT2 v2.2.1 Kim et al., 2019 http://daehwankimlab.github.io/ hisat2/ Feature Counts v2.0.0 Liao et al., 2014 http://subread.sourceforge.net/ Adobe Illustrator CS5 Adobe https://www.adobe.com/cn/ products/illustrator.html Other Fetal bovine serum GIBCO Cat# 10270106 Trypsin GIBCO Cat# 25200056 DMEM Hyclone Cat# SH30022.01B RPMI 1640 GIBCO Cat# 11875-093 Opti-MEM GIBCO Cat# 31985062 Trypsin-EDTA (0.25%), phenol red Invitrogen Cat# 25200056 Normal chow (NC) diet Shanghai shilin Bio. ..



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